S₁

Jablonski
Energy
Diagram

Ground State (S₀)

Solvent

Solvent

Solvent
Relaxed
S₁

Non-Polar
Solvent Not
Affected by
Fluorophore
Dipole

Fluorophore

100

80

60

40

20

0

Relative Intensity

300

400

500

600

700

Wavelength (Nanometers)

Absorption

Emission

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Start

Tutorial Speed

Solvent Polarity Low

High

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